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rabbit anti cpap  (Proteintech)


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    Proteintech rabbit anti cpap
    CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit <t>anti-CPAP,</t> and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.
    Rabbit Anti Cpap, supplied by Proteintech, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+cpap/pmc12861012-359-6-8?v=Proteintech
    Average 94 stars, based on 1 article reviews
    rabbit anti cpap - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Centrosomal P4.1-associated protein is a novel regulator of ESCRT pathway function during endosome maturation"

    Article Title: Centrosomal P4.1-associated protein is a novel regulator of ESCRT pathway function during endosome maturation

    Journal: iScience

    doi: 10.1016/j.isci.2026.114659

    CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit anti-CPAP, and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.
    Figure Legend Snippet: CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit anti-CPAP, and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.

    Techniques Used: Staining, Super-Resolution Microscopy, MANN-WHITNEY, Imaging



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    CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit <t>anti-CPAP,</t> and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.
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    Image Search Results


    CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit anti-CPAP, and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.

    Journal: iScience

    Article Title: Centrosomal P4.1-associated protein is a novel regulator of ESCRT pathway function during endosome maturation

    doi: 10.1016/j.isci.2026.114659

    Figure Lengend Snippet: CPAP was detected on endosomes in quiescent and EGF-treated cells, and its co-localization with ESCRT proteins increased during EVT HeLa cells were treated with untagged EGF for different time points, stained with AF488-linked sheep anti-EEA antibody, rabbit anti-CPAP, and mouse anti-HRS, or -TSG101 primary antibodies, followed by AF647- and AF568-linked anti-rabbit and -mouse secondary antibodies, and imaged using Lightning super-resolution microscopy. (A) Two-color co-localization. Left: single Z-plane of images showing EEA1 and CPAP staining of representative cells. Right: co-localization (yellow) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. (B) Three-color co-localization. Left: representative single Z-plane of images showing co-localization of CPAP, along with HRS or TSG101, on EEA1-positive puncta. Right: triple co-localization (white) was quantified by determining the percentages of EEA1-positive (green) puncta containing CPAP (red) and HRS/TSG101 (magenta) puncta in representative single Z-planes of each cell and quantified from multiple cells across at least three experiments. Zoomed images correspond to the dashed inset boxes of the indicated images. Scale bars: 10 μm. p values: ∗<0.05, ∗∗<0.01, ∗∗∗<0.001, ∗∗∗∗<0.0001 by unpaired nonparametric Mann-Whitney test. Note: (A) and (B) present data from the same experiments where 4-color imaging was done and convey information on two different aspects based on three or four markers at a time. Since the visuals of the same cell can help with more reliable interpretation of the data, images of the same cell were used, where possible, for (A) and (B) with the same or different pseudo-color. Hence, duplication of some sub-images among (A) and (B) is intentional.

    Article Snippet: Primary antibodies used in this study: rabbit anti-CPAP (Proteintech,11517-1-AP); mouse anti-CPAP (Abnova, H00055835-M01); anti-Rab5 (Proteintech, 20228-1-AP); anti-Rab7 (Proteintech, 55469-1-AP or Santa Cruz, sc-376362); Actin-HRP (Proteintech, 60008-1-Ig); -GFP (Proteintech, 66002-1-Ig or 50430-2-AP); -HRS (Santa Cruz, sc-271455); -TSG101 (Santa Cruz, sc-7964; Proteintech, 28283-1-AP); and -ALIX (Biolegend, 634502), EEA1 (Invitrogen, PA1-0337 or BD biosciences 610456) and CD63 (BD biosciences, 556019).

    Techniques: Staining, Super-Resolution Microscopy, MANN-WHITNEY, Imaging

    Journal: Cell

    Article Title: Time-series reconstruction of the molecular architecture of human centriole assembly

    doi: 10.1016/j.cell.2024.03.025

    Figure Lengend Snippet:

    Article Snippet: Rabbit polyclonal anti-CPAP , Proteintech , Cat# 11517-1-AP, RRID: AB_2244605.

    Techniques: Recombinant, Software